Optimal dilutions for each application to be determined by the researcher
Anwendungsbeschreibung:
Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Flow analysis of permeablized PC3 cells using ODC-1 antibody.
Immunofluorescence testing of LNCaP cells and Alexa Fluor 488 conjugated ODC-1 antibody.
Formalin/paraffin human prostate carcinoma stained with ODC-1 antibody.
Formalin/paraffin rat pancreas stained with ODC-1 antibody.
Formalin/paraffin human testicular carcinoma stained with ODC-1 antibody.
Western blot testing of human samples using ODC-1 mAb.
SDS-PAGE Analysis of Purified, BSA-Free ODC-1 Antibody (clone ODC1/485). Confirmation of Integrity and Purity of the Antibody.
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