Histone Antibody [1415-1], Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: Histone Antibody [1415-1], Unconjugated, Mouse, Monoclonal
• Artikelnummer: PRS-33-387
• Hersteller Artikelnummer: 33-387
• Alternativnummer: PRS-33-387-100
• Hersteller: ProSci
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.
• Konjugation: Unconjugated
• Alternative Synonym: H1F0, H10, H1FV, MGC5241

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.2 mg/mL
• Klon-Bezeichnung: [1415-1]
• NCBI: 3005
• UniProt: P07305
• Puffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher
• Anwendungsbeschreibung: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Histone antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Bilder

Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1)

Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (1415-1)

FFPE human testicular carcinoma tested with Histone H1 antibody (1415-1)

FFPE human pancreas tested with Histone H1 antibody (1415-1)

FFPE human ovarian carcinoma tested with Histone H1 antibody (1415-1)

FFPE human angiosarcoma tested with Histone H1 antibody (1415-1)

Western blot testing of HeLa cell lysate (nuclear fraction) with Histone antibody (clone 1415-1). Observed molecular weight ~22/30-33kDa (unmodified/modified).