Neuron Specific Enolase Antibody [ENO2/1375], IgG2b, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: Neuron Specific Enolase Antibody [ENO2/1375], IgG2b, Unconjugated, Mouse, Monoclonal
• Artikelnummer: PRS-33-754
• Hersteller Artikelnummer: 33-754
• Alternativnummer: PRS-33-754-100
• Hersteller: ProSci
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.
• Konjugation: Unconjugated
• Alternative Synonym: ENO2, Enolase 2, Enolase 2 (gamma, neuronal), Neurone-specific enolase, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.2 mg/mL
• Klon-Bezeichnung: [ENO2/1375]
• Isotyp: IgG2b
• NCBI: 2026
• UniProt: P09104
• Puffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher
• Anwendungsbeschreibung: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 ug/mlIHC (FFPE): 0.1-0.2 ug/ml for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.

Bilder

IHC testing of FFPE human cerebellum with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE human pheochromocytoma with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE mouse pancreas with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE rat heart with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

Western blot testing of human 1) Y79, 2) HeLa and 3) HepG2 cell lysate with NSE antibody (clone ENO2/1375). Predicted molecular weight ~47 kDa.

SDS-PAGE Analysis of Purified, BSA-Free NSE Antibody (clone ENO2/1375). Confirmation of Integrity and Purity of the Antibody.