ATF6 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ATF6 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-3683
• Hersteller Artikelnummer: 3683
• Alternativnummer: PRS-3683-0.02,PRS-3683-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: ATF6 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6.The immunogen is located within amino acids 30 - 80 of ATF6.
• Konjugation: Unconjugated
• Alternative Synonym: ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL
• NCBI: 22926
• UniProt: P18850
• Puffer: ATF6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 0.5-2 µg/mL, IHC: 5 µg/mLAntibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

Bilder

Figure 2 WB Validation in Human Cell Lines Loading: 15 &956,g of human cell lysate Antibodies ATF6 3683, 1 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.conjugate at 1:10000 dilution.

Figure 3 WB Validation in Mouse Tissue Loading: 15 &956,g of mouse tissue lysate Antibodies: ATF6 3683, 1 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 5 Immunohistochemistry Validation of ATF6 in Human Testis Tissue Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ATF6 antibody (3683) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 6 Immunohistochemistry Validation of ATF6 in Mouse Kidney Tissue Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATF6 antibody (3683) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 7 Immunohistochemistry Validation of ATF6 in Rat Kidney Tissue Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ATF6 antibody (3683) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 1 KO Validation of ATF6 Loading: 15 &956,g of human HeLa cell lysate Antibodies ATF6 3683, 0.5 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 WB Validation in Rat Heart Loading: 15 &956,g of rat kidney lysate Antibodies: ATF6 3683, 1 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.