TWEAK Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: TWEAK Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-3791
• Hersteller Artikelnummer: 3791
• Alternativnummer: PRS-3791-0.02,PRS-3791-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC-P, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Anti-TWEAK antibody (3791) was raised against recombinant human TWEAK protein.
• Konjugation: Unconjugated
• Alternative Synonym: TWEAK Antibody: APO3L, DR3LG, TWEAK, APO3L, UNQ181/PRO207, Tumor necrosis factor ligand superfamily member 12, APO3 ligand

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL
• Molekulargewicht: Predicted: 27 and 36 kDObserved: 27 and 36 kD
• NCBI: 8742
• UniProt: O43508
• Puffer: TWEAK Antibody is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 0.5-4 µg/mL, IHC: 5-10 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human and mouse samples, Immunofluorescence in mouse samples. All other applications and species not yet tested.

Bilder

Figure 1 Western Blot Validation in HeLa Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: TWEAK 3791 (A: 1 &956,g/mL and B: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 6 Immunofluorescence Validation of TWEAK in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TWEAK with 3791 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Figure 5 Immunohistochemistry Validation of TWEAK in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-TWEAK antibody (3791) at 10 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 6 Immunohistochemistry Validation of TWEAK in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TWEAK antibody (3791) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 2 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human TWEAK recombinant protein per lane.Antibodies: TWEAK 3791 (Lane 1: 0.5 &956,g/mL, Lane 2: 1 &956,g/mL, Lane 3: 2 &956,g/mL and Lane 4: 4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 18kD.

Figure 3 Western Blot Validation in Mouse Pancreas Tissue LysatesLoading: 15 &956,g of lysates per lane.Antibodies: TWEAK 3791 (Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 Western Blot Validation in Mouse Liver Tissue LysatesLoading: 15 &956,g of lysates per lane.Antibodies: TWEAK 3791 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.