Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant h4-1BBL (human 4-1BBLigand). Human 4-1BBL specific antibody was purified by affinity chromatography and then biotinylated.
ELISA:Sandwich:To detect h4-1BBL by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human 4-1BBL as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant h4-1BBL. Western Blot:To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant h4-1BBL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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