TPO Antibody (biotin), Biotin, Rabbit, Polyclonal

Artikelnummer: PRS-38-325
Artikelname: TPO Antibody (biotin), Biotin, Rabbit, Polyclonal
Artikelnummer: PRS-38-325
Hersteller Artikelnummer: 38-325
Alternativnummer: PRS-38-325-0.025
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTPO. Anti-Human TPO specific antibody was purified by affinity chromatography and then biotinylated.
Konjugation: Biotin
Alternative Synonym: Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF
Klonalität: Polyclonal
Konzentration: batch dependent
NCBI: 7066
UniProt: P40225
Formulierung: Lyophilized
Application Verdünnung: Centrifuge vial prior to opening.
Anwendungsbeschreibung: Sandwich ELISA: To detect hTPO by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProScis Polyclonal Anti-Human TPO as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO. Western Blot To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing condition