Produced from sera of rabbits immunized with highly pure recombinant Rat IL-13. Anti-Rat IL-13-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Rat IL-13 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProScis Polyclonal Anti- Rat IL-13 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-13. Western Blot To detect Rat IL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat IL-13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten