CLPB Antibody, Unconjugated, Goat, Polyclonal

Artikelnummer: PRS-46-646
Artikelname: CLPB Antibody, Unconjugated, Goat, Polyclonal
Artikelnummer: PRS-46-646
Hersteller Artikelnummer: 46-646
Alternativnummer: PRS-46-646-0.1
Hersteller: ProSci
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA
Immunogen: The immunogen for this antibody is: C-DKDSKTRRLDIRAP
Konjugation: Unconjugated
Alternative Synonym: CLPB, ClpB caseinolytic peptidase B homolog (E. coli), FLJ13152, HSP78, SKD3, suppressor of potassium transport defect 3
Klonalität: Polyclonal
Konzentration: 500 ug/mL
NCBI: 81570
UniProt: Q9H078
Puffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung: Liquid
Anwendungsbeschreibung: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 50kDa and 48kDa in lysates of HeLa and HepG2 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 78.7kDa according to NP_110440.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?