IL-17 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: IL-17 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-4887
• Hersteller Artikelnummer: 4887
• Alternativnummer: PRS-4887-0.02,PRS-4887-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC-P, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Anti-IL-17 antibody (4887) was raised against a peptide corresponding to 16 amino acids near the center of mature human IL-17. The immunogen is located within amino acids 50-100 of IL-17.
• Konjugation: Unconjugated
• Alternative Synonym: IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL
• Molekulargewicht: Predicted: 17.5kDObserved: 18 kDa
• NCBI: 3605
• UniProt: Q16552
• Puffer: IL-17 Antibody is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 0.125-2 µg/mL, IF: 5-20 µg/mL, IHC: 1-5 µg/mL .Antibody validated: Western Blot in human samples, Immunofluorescence in mouse samples, Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.

Bilder

Figure 5 Immunofluorescence Validation of IL-17 in Mouse A-20 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse A-20 Cells labeling IL-17 with 4887 at 5 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 6 Immunofluorescence Validation of IL-17 in Mouse Thymus TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17 with 4887 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Figure 4 Immunofluorescence Validation of IL-17 in Mouse Heart TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart issue labeling IL-17 with 4887 at 10 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 8 Immunohistochemistry Validation of IL-17 in Human Tonsil Tissue Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-17 antibody (4887) at 5 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730, C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 7 Immunohistochemistry Validation of IL-17 in Mouse Colon Tissue Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL-17 antibody (4887) at 2 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730, C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 3 Immunohistochemistry Validation of IL-17 in Human Lymph Node Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-IL-17 antibody (4887) at 1 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 1 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human IL-17 recombinant protein per lane.Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.125 &956,g/mLLane 2: 0.25 &956,g/mLLane 3: 0.5 &956,g/mL

Figure 2 Western Blot Validation with Human Spleen Loading: 10 &956,g of lysates per lane.Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 minLane 1: 1 &956,g/mL Lane 2: 2 &956,g/mL