NANOG Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: NANOG Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-5585
• Hersteller Artikelnummer: 5585
• Alternativnummer: PRS-5585-0.02,PRS-5585-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG.The immunogen is located within amino acids 130 - 180 of NANOG.
• Konjugation: Unconjugated
• Alternative Synonym: NANOG Antibody: Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL
• Molekulargewicht: Predicted: 35 kD Observed: 35 kD
• NCBI: 79923
• UniProt: Q9H9S0
• Puffer: NANOG Antibody is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1-2 µg/mL, ICC/IF: 20 µg/mL, IHC: 1-5 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human, mouse and rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Bilder

Figure 2 WB Validation in HeLa CellsLoading: 15 &956,g of lysate. Antibodies: NANOG 5585, 2 &956,g/mL , 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.

Figure 3 Western Blot Validation in Mouse and Rat Testis Loading: 15 &956,g of lysates per lane. Antibodies: NANOG 5585, 2 &956,g/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Figure 1 Immunoflouorescence Validation of NANOG in HeLa CellsImmunofluorescent analysis of PFA-fixed HeLa cells labeling NANOG with 5585 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

Figure 4 Immunohistochemistry Validation of NANOG in Human Testis Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-NANOG antibody (5585) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 5 Immunohistochemistry Validation of NANOG in Mouse Testis Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NANOG antibody (5585) at 2 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 6 Immunohistochemistry Validation of NANOG in Rat Testis Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NANOG antibody (5585) at 2 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.