DVL1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: DVL1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-56-349
• Hersteller Artikelnummer: 56-349
• Alternativnummer: PRS-56-349-400
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-P, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: This DVL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-470 amino acids from the Central region of human DVL1.
• Konjugation: Unconjugated
• Alternative Synonym: Segment polarity protein dishevelled homolog DVL-1, Dishevelled-1, DSH homolog 1, DVL1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: 75 kDa
• NCBI: 1855
• UniProt: O14640
• Puffer: Supplied in PBS with 0.09% (W/V) sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: For IF starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000

Bilder

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Antibody staining DVL1 in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MDA-MB-453 whole cell lysate Lane 3: PC-3 whole cell lysate Lane 4: human kidney lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution + PC-3 whole cell lysates Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: MDA-MB-453 whole cell lysates Lane 3: PC-3 whole cell lysates Lane 4: human kidney lysates Lysates/proteins at 20 ug per lane.

Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).