UCHL1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: UCHL1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-61-087
• Hersteller Artikelnummer: 61-087
• Alternativnummer: PRS-61-087-400
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, IF, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: This UCHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from the C-terminal region of human UCHL1.
• Konjugation: Unconjugated
• Alternative Synonym: Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, 6---, Neuron cytoplasmic protein 95, PGP 95, PGP95, Ubiquitin thioesterase L1, UCHL1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: 25 kDa
• NCBI: 7345
• UniProt: P09936
• Puffer: Supplied in PBS with 0.09% (W/V) sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50

Bilder

Antibody staining UCHL1 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Antibody staining UCHL1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Antibody staining UCHL1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western blot analysis in U266,SH-SY5Y,NCI-H1299,mouse Neuro-2a,rat C6,PC-12 cell line and mouse brain tissue lysates (35ug/lane).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma, HC = hepatocarcinoma.

Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of UCHL1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).