This CD63 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-190 amino acids from the C-terminal region of human CD63.
Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
For IHC-P starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000
Antibody staining CD63 in human melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Overlay histogram showing HL-60 cells stained with Antibody (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10 6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution + human plasma lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: A2058 whole cell lysates Lane 2: A375 whole cell lysates Lane 3: HL-60 whole cell lysates Lane 4: THP-1 whole cell lysates Lane 5: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: A2058 whole cell lysates Lane 2: MDA-MB-453 whole cell lysates Lysates/proteins at 20 ug per lane.
CD63 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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