KLOTHO Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: KLOTHO Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-6107
• Hersteller Artikelnummer: 6107
• Alternativnummer: PRS-6107-0.02,PRS-6107-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Anti-KLOTHO antibody (6107) was raised against a peptide corresponding to 17 amino acids near the center of human KLOTHO. The immunogen is located within amino acids 410-460 of KLOTHO.
• Konjugation: Unconjugated
• Alternative Synonym: KLOTHO Antibody: Klotho

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL
• Molekulargewicht: Predicted: 116kDObserved: 116kD
• NCBI: 9365
• UniProt: Q9UEF7
• Puffer: KLOTHO Antibody is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1-4 µg/mL, IHC: 2.5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in mouse and rat samples, Immunofluorescence in mouse samples. All other applications and species not yet tested.

Bilder

Figure 5 Immunofluorescence Validation of KLOTHO in Mouse Heart TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling KLOTHO with 6107 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Figure 6 Immunohistochemistry Validation of KLOTHO in Rat Heart Tissue Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-KLOTHO antibody (6107) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 4 Immunohistochemistry Validation of KLOTHO in Mouse Heart Tissue Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-KLOTHO antibody (6107) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: KLOTHO 6107 (2 &956,g/mL), KLOTHO, competitor antibody (4 &956,g/mL) and beta-actin 3779 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 2 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human KLOTHO recombinant protein per lane.Antibodies: KLOTHO 6107 (Lane 1: 1 &956,g/mL, Lane 2: 2 &956,g/mL and Lane 3: 4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 72kD.

Figure 3 Western Blot Validation in Rat Kidney Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: KLOTHO 6107 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.