This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.
Konjugation:
Unconjugated
Alternative Synonym:
Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SPK
Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
Antibody staining SPHK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining SPHK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301) at 1:1000 at each lane.
Antibody is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.
Western blot analysis of anti-SPHK1 Antibody in 293 cell line lysates (35ug/lane)
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