This ANXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human ANXA2.
Konjugation:
Unconjugated
Alternative Synonym:
Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, PAP-IV, Protein I, p36, ANXA2, ANX2, ANX2L4, CAL1H, LPC2D
Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
Antibody staining ANXA2 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lane 4: SH-SY5Y whole cell lysates Lane 5: SK-BR-3 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of ANXA2 using rabbit polyclonal ANXA2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ANXA2 gene.
Western blot analysis of anti-ANXA2 Antibody in mouse lung tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in 293,MDA-MB231 cell line lysates (35ug/lane).
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