This CDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-305 amino acids from the C-terminal region of human CDK4.
Konjugation:
Unconjugated
Alternative Synonym:
Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4
Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
Fluorescent image of Hela cell stained with CDK4 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDK4 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).CDK4 immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis of anti-CDK4 Pab in HL-60 cell lysate
Western blot analysis of CDK4 using rabbit polyclonal CDK4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK4 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma, HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human prostate carcinoma with CDK4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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