This EphA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-60 amino acids from the N-terminal region of human EphA2.
Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100
Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of hEPHA2-T45 in MCF-7 cell line lysates (35ug/lane)
Western blot analysis of hEPHA2-T45 in mouse NIH-3T3 cell line lysates (35ug/lane)
Western blot analysis of EPHA2 using rabbit polyclonal hEPHA2-T45 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA2 gene (Lane 2)
Flow cytometric analysis of NCI-H292 cells using EphA2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma, HC = hepatocarcinoma.
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