TrkA-pY791 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: TrkA-pY791 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-63-142
• Hersteller Artikelnummer: 63-142
• Alternativnummer: PRS-63-142-400
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, IHC-P, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: This TrkA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 769-796 amino acids from human TrkA.
• Konjugation: Unconjugated
• Alternative Synonym: High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, Trk-A, gp140trk, p140-TrkA, NTRK1, MTC, TRK, TRKA

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: 87 kDa
• NCBI: 4914
• UniProt: P04629
• Puffer: Supplied in PBS with 0.09% (W/V) sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000

Bilder

Overlay histogram showing SH-SY5Y cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10 6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Antibody staining TrkA in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution + mouse brain lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of hTrkA-pY791 in HepG2 cell line lysates (35ug/lane)

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

TrkA-pY791 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.