This NEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-426 amino acids from the Central region of human NEK2.
Konjugation:
Unconjugated
Alternative Synonym:
Serine/threonine-protein kinase Nek2, HSPK 21, Never in mitosis A-related kinase 2, NimA-related protein kinase 2, NimA-like protein kinase 1, NEK2, NEK2A, NLK1
Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
For IHC-P starting dilution is: 1:100For IF starting dilution is: 1:25For WB starting dilution is: 1:1000
Immunohistochemical analysis of paraffin-embedded H. brain section using NEK2 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Fluorescent image of U251 cells stained with hNEK2-C410 . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded R. brain section using NEK2 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. brain section using NEK2 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from 293T, Hela, Jurkat, K562, MCF-7, and mouse NIH/3T3 cell line (from left to right), using NEK2 Antibody (C410) at 1:1000 at each lane.
Western blot analysis of anti-NEK2 Antibody in HL60 cell line lysates (35ug/lane)
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