This STAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 717-745 amino acids from the C-terminal region of human STAT1.
Konjugation:
Unconjugated
Alternative Synonym:
Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1
Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:1000For IF starting dilution is: 1:25For FACS starting dilution is: 1:25
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling STAT1 with 64-232 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus and Weak Cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human brain tissue using 64-232 performed on the Leica BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human lymph node tissue using 64-232 performed on the Leica BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 64-232 performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Overlay histogram showing Hela cells stained with 64-232(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (64-232, 1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10 6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-STAT1 Antibody (C-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: Daudi whole cell lysate Lane 5: Hela whole cell lysate Lane 5: MCF-7 whole cell lysate Lane 5: HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 87 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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