SARS-CoV-2 (COVID-19) Spike 681P Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SARS-CoV-2 (COVID-19) Spike 681P Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-9091
• Hersteller Artikelnummer: 9091
• Alternativnummer: PRS-9091-0.02,PRS-9091-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Spezies Reaktivität: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site) (9091) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
• Konjugation: Unconjugated
• Alternative Synonym: SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site): Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, covid-19, sars-cov-2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL
• NCBI: 43740568
• UniProt: P0DTC2
• Puffer: SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site) is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 0.5-4 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human samples, Immunofluorescence in human samples. SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site) can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in ELISA. It will detect 4 ng of free peptide at 1 µg/mL. Antibody can also be used for as capture antibody in Sandwich Elisa at 10 µg/mL. All other applications and species not yet tested.

Bilder

Figure 5 ELISA Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant ProteinAntibodies: SARS-CoV-2 (COVID-19) Spike antibody (cleavage site), 9091 (1 &956,g/mL). A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S1 recombinant protein (97-087) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike antibody (cleavage site) as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.

Figure 7 ELISA Validation with SARS-CoV-2 (COVID-19) Spike Recombinant ProteinAntibodies: SARS-CoV-2 (COVID-19) Spike antibody (cleavage site), 9091. A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike RBD+SD1+SD2 recombinant protein, 10-305, as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike antibody (cleavage site) as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000ng/mL.

Figure 8 ELISA Validation of SARS-CoV-2 Spike Antibody PairA sandwich ELISA was performed using the anti-SARS-COV-2 Spike S1 antibodies 9091 (10 &956,g/mL ) as the capture antibody. Biotin-labled anti-SARS-COV-2 Spike S1 antibodies 9083-biotin (1 &956,g/mL ) and streptavidin-HRP (0.1 &956,g/mL ) were used for detection. Detection range is from 0.8 ng to 200 ng (SARS-CoV-2 Spike S1 Protein, 97-087).

Figure 1 SARS-Cov-2 Spike 681P Antibodies Specifically Do Not Recognize Omicron Variant Spike S1 Protein in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Spike 681P antibody, 9091, 1 &956,g/mL,, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.SARS-CoV-2 spike 681P antibody (9091) can detect spike S1 protein of all other variants, but not omicron variant.

Figure 2 Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Spike (cleavage site) in 293 Transfected CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Spike transfected 293cells labeling SARS-CoV-2 (COVID-19) Spike (cleavage site) with 9091 at 10 mg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 3 Overexpression Validation in Spike Transfected 293 Cells Loading: 10 &956,g per lane of 293 cell lysate. Antibodies: SARS-CoV-2 (COVID-19) Spike (cleavage site), 9091 (0.5 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells

Figure 4 Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein Loading: 50 ng per lane of SARS-CoV-2 (COVID-19) Spike S1 recombinant protein (97-087). Antibodies: SARS-CoV-2 (COVID-19) Spike (cleavage site), 9091, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 &956,g/mL and Lane 2: 2 &956,g/mL.

Figure 6 Western Blot Validation with SARS-CoV-2 (COVID-19) Spike Recombinant Protein Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike RBD+SD1+SD2 recombinant protein, 10-305. Antibodies: SARS-CoV-2 (COVID-19) Spike (cleavage site), 9091, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 &956,g/mL, Lane 2: 2 &956,g/mL and Lane 3: 4 &956,g/mL.