SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-9153
• Hersteller Artikelnummer: 9153
• Alternativnummer: PRS-9153-0.02,PRS-9153-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IHC, WB
• Spezies Reaktivität: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody (9153) was raised against a peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein.
• Konjugation: Unconjugated
• Alternative Synonym: 3C-like proteinase, 3CLp, Main protease, Mpro, Non-structure protein 5, covid-19, nsp5, sars-cov-2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL
• NCBI: 43740578
• UniProt: P0DTC1
• Puffer: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: IHC: 0.5 µg/mL, WB: 0.5 µg/mLAntibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can detect 2 ng of free peptide at 1 µg/mL in ELISA. It can detect SARS-CoV-2 NSP5 recombinant protein by ELISA and WB. All other applications and species not yet tested.

Bilder

Figure 3 Western Blot Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) recombinant protein (10-400).Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5), 9153, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.5 &956,g/mL and Lane 2: 1 &956,g/mL

Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, 9153. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein (10-400) as coating antigen and the anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL

Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) 3L-PRO in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) 3L-PRO (NSP5) antibody (9153, 0.5 &956,g/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 3L-PRO (NSP5) protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.