SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-9181
• Hersteller Artikelnummer: 9181
• Alternativnummer: PRS-9181-0.02,PRS-9181-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IHC
• Spezies Reaktivität: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody (9181) was raised against a peptide corresponding to 19 amino acids near the carboxyl terminus of the SARS-CoV-2 (COVID-19) NSP13 (Helicase) protein. The immunogen is located in the last 50 amino acids of SARS-CoV-2 (COVID-19) NSP13 (Helicase).
• Konjugation: Unconjugated
• Alternative Synonym: Helicase, NSP13, Non-structural protein 13, covid-19, sars-cov-2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL
• NCBI: 43740578
• UniProt: P0DTC1
• Puffer: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: IHC: 0.5 µg/mL.Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP13 antibody can detect 2 ng of free peptide at 1 µg/mL in ELISA. It can detect SARS-CoV-2 NSP13 recombinant protein by ELISA. All other applications and species not yet tested

Bilder

Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP13 (Helicase) Protein Antibodies: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, 9181. A direct ELISA was performed using SARS-CoV-2 NSP13 recombinant protein (10-427) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.

Figure 3 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, 9181. A direct ELISA was performed using immunogen peptide (9181P) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.

Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP13 in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP13 antibody (9181, 0.5 &956,g/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP13 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.