SARS-CoV-2 (COVID-19) ORF3b Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SARS-CoV-2 (COVID-19) ORF3b Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-9281
• Hersteller Artikelnummer: 9281
• Alternativnummer: PRS-9281-0.02,PRS-9281-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Virus
• Immunogen: Anti-SARS-CoV-2 (COVID-19) ORF3b antibody (9281) was raised against a peptide corresponding to 13 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF3b protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF3b protein.
• Konjugation: Unconjugated
• Alternative Synonym: Acessary protein 3b, Non-structural protein 3b, ORF3b, ORF3b protein, covid-19, ns3b, sars-cov-2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL
• Molekulargewicht: 17kD
• NCBI: 43740569
• UniProt: P0DTC6
• Puffer: SARS-CoV-2 (COVID-19) ORF3b antibody is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 0.25 µg/mLAntibody validated: SARS-CoV-2 (COVID-19) ORF3b antibody can detect 2 ng of free peptide at 1 µg/mL in ELISA. It can detect SARS-CoV-2 ORF3b recombinant protein by ELISA and WB. All other applications and species not yet tested.

Bilder

Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) ORF3b Protein Antibodies: SARS-CoV-2 (COVID-19) ORF3b Antibody, 9281. A direct ELISA was performed using SARS-CoV-2 ORF3b recombinant protein (10-005) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF3b antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL

Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF3b ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF3b recombinant protein (10-005).Antibodies: SARS-CoV-2 (COVID-19) ORF3b, 9281, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 &956,g/mL and Lane 2: 0.5 &956,g/mL