SARS-CoV-2 (COVID-19) Spike 26P Antibody [1C3H9], IgG2b, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: SARS-CoV-2 (COVID-19) Spike 26P Antibody [1C3H9], IgG2b, Unconjugated, Mouse, Monoclonal
• Artikelnummer: PRS-PM-9583
• Hersteller Artikelnummer: PM-9583
• Alternativnummer: PRS-PM-9583-0.02,PRS-PM-9583-0.1
• Hersteller: ProSci
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Virus
• Immunogen: Anti-SARS-CoV-2 Spike 26P antibody (PM-9583) was raised against a peptide corresponding to 11 amino acids near the amino terminus of SARS-CoV-2 Spike protein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 Spike protein.
• Konjugation: Unconjugated
• Alternative Synonym: SARS-CoV-2 Spike 156-157EF antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, covid-19, sars-cov-2

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/mL
• Klon-Bezeichnung: [1C3H9]
• Isotyp: IgG2b
• NCBI: 43740568
• UniProt: P0DTC2
• Puffer: SARS-CoV-2 Spike 26P antibody is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1 µg/mL. Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike 26P antibody detects all other SARS-CoV-2 variant Spike S1 protein, but not SARS-CoV-2 Gamma variant Spike S1 protein by ELISA. All other applications and species not yet tested.

Bilder

Figure 2 ELISA Validation of Spike 26P Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein Coating Antigen: SARS-CoV-2 spike S1 proteins of WT and Gamma variant (P.1), 1 &956,g/mL, incubate at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Spike 26P antibody, PM-9583, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike 26P antibody detects WT spike S1 protein (10-300), but not Gamma variant spike S1 protein.

Figure 4 ELISA Validation of Spike 26P Antibodies with Mutant and WT Peptide Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (9083P) and Gamma variant (P.1) (9573P), 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 26P antibody, PM-9583, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 spike 26P antibody detects WT peptide (26P, 9083P), but not Gamma variant spike S1 peptide (26S, 9573P).

Figure 1 SARS-Cov-2 Spike 26P Antibodies Detect Spike S1 Proteins of all Variants except Gamma Variant in an ELISA Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 &956,g/mL, incubated at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Spike 26P antibody, PM-9583, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.

Figure 3 ELISA Validation of Spike 26P Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, WT and Gamma variant (P.1).Detection Antibodies: Spike 26P antibody, PM-9583, 1 &956,g/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike 26P antibody detects WT spike S1 protein (10-300), but not Gamma variant spike S1 protein.