PDL1 Antibody [8E12], IgG1, Unconjugated, Mouse, Monoclonal

Artikelnummer: PRS-RF16032
Artikelname: PDL1 Antibody [8E12], IgG1, Unconjugated, Mouse, Monoclonal
Artikelnummer: PRS-RF16032
Hersteller Artikelnummer: RF16032
Alternativnummer: PRS-RF16032-0.02,PRS-RF16032-0.1
Hersteller: ProSci
Wirt: Mouse
Kategorie: Antikörper
Applikation: ELISA, FC, ICC, IF, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: PD-L1 antibody was raised against the extracellular domain of human PD-L1.
Konjugation: Unconjugated
Alternative Synonym: PD-L1 Antibody: Programmed cell death 1 ligand-1, programmed death ligand 1, PDL1, PDL-1, B7-H1
Klonalität: Monoclonal
Konzentration: 1 mg/mL
Klon-Bezeichnung: [8E12]
Molekulargewicht: Predicted: 32 kDa Observed: 45 kDa
Isotyp: IgG1
NCBI: 29126
UniProt: Q9NZQ7
Puffer: PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.25 - 0.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 µg/mL. For immunofluorescence start at 5 µg/mL.Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
Flow cytometry analysis of PD-L1 overexpressing HEK293 cells using PD-L1 antibody and control mouse IgG antibody at 10 &956,g/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L1 overexpressing HEK293 cells.
Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 &956,g/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 &956,g/mL.
Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 &956,g/mL. Red: PDL1 Antibody [8E12] (RF16032) Blue: DAPI staining
Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 &956,g/mL. Red: PDL1 Antibody [8E12] (RF16032) Blue: DAPI staining
Immunofluorescence of PD-L1 in human tonsil tissue with PD-L1 antibody at 2 &956,g/mL. Red: PDL1 Antibody [8E12] (RF16032) Blue: DAPI staining
Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 5 &956,g/mL.
Immunohistochemistry of PD-L1 in human tonsil tissue with PD-L1 antibody at 5 &956,g/mL.
Western blot analysis of PD-L1 in overexpressing HEK293 cells PD-L1 antibody at 0.25 and 0.5 &956,g/ml