flt3-Ligand Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: flt3-Ligand Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-XP-5138
• Hersteller Artikelnummer: XP-5138
• Alternativnummer: PRS-XP-5138-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, NeA, WB
• Spezies Reaktivität: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hflt3-Ligand.
• Konjugation: Unconjugated
• Alternative Synonym: FL, FLT3LFms-related tyrosine kinase 3 ligand, SL cytokine, Flt3 ligand

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• NCBI: 2323
• UniProt: P49771
• Formulierung: Lyophilized

Anwendungsbeschreibung

• Application Verdünnung: Centrifuge vial prior to opening.
• Anwendungsbeschreibung: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hflt3- Ligand (15 ng/mL), a concentration of 0.25 - 0.75 µg/mL of this antibody is required. ELISA:To detect hflt3-Ligand by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hflt 3-Ligand. Sandwich: To detect hFGF-basic by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human FGF-basic (XP-5138Bt) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-basic.Western Blot:To detect hflt3-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hflt3-Ligand is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.