IL-7 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: IL-7 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-XP-5197
• Hersteller Artikelnummer: XP-5197
• Alternativnummer: PRS-XP-5197-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, NeA, WB
• Spezies Reaktivität: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-7 (human Interleukin-7).
• Konjugation: Unconjugated
• Alternative Synonym: IL-7Interleukin-7IL-7

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• NCBI: 3574
• UniProt: P13232
• Formulierung: Lyophilized

Anwendungsbeschreibung

• Application Verdünnung: Centrifuge vial prior to opening.
• Anwendungsbeschreibung: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-7 (1.00 ng/mL), a concentration of 0.016 0.03 µg/mL of this antibody is required. ELISA:To detect hIL-7 by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-7. Sandwich:To detect hIL-7 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-7 (XP-5197Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-7. Western Blot:To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.