Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hNeurturin (50.00 ng/mL), a concentration of 0.5 - 0.75 µg/mL of this antibody is required. ELISA:To detect hNeurturin by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNeurturin. Sandwich:To detect hNeurturin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Neurturin (38-115) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNeurturin.Western Blot:To detect hNeurturin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNeurturin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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