ELISA:In a sandwich ELISA (assuming 100 µL/well), a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 1000 pg/mL of recombinant human MIG when used with our biotinylated antigen affinity purified anti-human MIG (XP-5231Bt) as the detection antibody at a concentration of approximately 1.0-2.0 µg/mL. Sandwich:To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.50-2.0 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 0.25-0.50 ng/lane, under non-reducing conditions and 2.0-4.0 ng/lane, under reducing conditions.
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