Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1alpha (100 ng/mL), a concentration of 5.0-10.0 µg/mL of this antibody is required.ELISA:In a sandwich ELISA (assuming 100 µL/well), a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1alpha when used with our biotinylated antigen affinity purified anti-human MIP-1alpha (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 µg/mL.Western Blot:To detect hMIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1alpha is 1.0-2.0 ng/lane, under non-reducing conditions.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten