The affinity purified antibodies react only with chicken IgY and not IgM or IgA immunoglobulins by immunodiffusion and IEP techniques. In a sandwich ELISA against 1 µg/mL chicken IgY, conjugated antibody diluted to 1:100,000 produced an O.D. = 1.0 in a 15 minute reaction with TMB substrate. Recommended Western Blot dilution: 1:2,000 - 1:4,000 when used for colorimetric assay, and 1:10,000 or up for ECL detection system. Optimal conjugate working dilutions should be determined for your particular assay conditions.
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