Recombinant human Troponin T, cardiac muscle/cTNT overexpression cell lysate, derived from the transfected HEK293 cells. (100 µg). Purchase will also include one vial of normal control HEK293 cell lysate (Catalog 230-10006) transfected with empty expression vector.
Konzentration:
Determined by BCA protein assay kit (Thermo Scientific)
Molekulargewicht:
Recombinant protein has a calculated molecular mass of 33. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).
Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Formulierung:
Lyophilized powder
Sequenz:
Ser2-Lys298
Formel:
Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail
Anwendungsbeschreibung:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
