mouse Nanog, transfected HEK293 cell lysate, ES cell-associated protein 4, Early embryo specific expression NK-type homeobox protein, Homeobox transcription factor Nanog.
Recombinant mouse Homeobox protein NANOG overexpression cell lysate, derived from the transfected HEK293 cells (100 µg). Purchase will also include one vial of normal control HEK293 cell lysate (catalog . 230-10006) transfected with empty expression vector.
Konzentration:
Determined by Pierce BCA protein assay kit
Molekulargewicht:
Recombinant protein has a calculated molecular mass of 34. The actual molecular weight may increase differently due to the potential post-modifications (PTMs).
Tag:
His
Expression System:
HEK293 cells
Reinheit:
Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Formulierung:
Lyophilized powder
Sequenz:
Ser2-Ile305
Formel:
Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail
Anwendungsbeschreibung:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
