Recombinant human creatine kinase B (CKB) protein overexpression cell lysate, derived from the transfected HEK293 cells. Purchase will also include one vial of normal control (Catalog . 230-10006), the cell lysate of HEK293 cells transfected with empty expression vector.
Konzentration:
Determined by BCA protein assay
Molekulargewicht:
Recombinant protein product has a calculated molecular mass of 43 kDa.
Tag:
His
Expression System:
HEK293 cells
Reinheit:
Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Formulierung:
Lyophilized powder
Sequenz:
Pro2-Lys381
Formel:
Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) containing mammalian cell protease inhibitor cocktail
Anwendungsbeschreibung:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
