Recombinant human RANKL overexpressed in cell culture supernatant (serum free), derived from transfected HEK293 cells (200 µl). Purchase will also include one vial of normal control, the culture supernatant of HEK293 cell transfected with empty expression vector.
Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Formulierung:
Pink liquid
Sequenz:
Ile140-Asp317
Formel:
Culture supernatant of transfected HEK293 cells
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
