Chicken IgG Alkaline Phosphatase, ALP

Artikelnummer: ROC-003-0502
Artikelname: Chicken IgG Alkaline Phosphatase, ALP
Artikelnummer: ROC-003-0502
Hersteller Artikelnummer: 003-0502
Alternativnummer: ROC-003-0502
Hersteller: Rockland Immunochemicals
Kategorie: Antikörper
Applikation: DOT
Konjugation: ALP
Alternative Synonym: Chicken IgG alkaline phosphatase conjugated, Chicken IgG alk phos conjugated, Chicken IgY alkaline phosphatase conjugated, Chicken IgY alk phos conjugated
Konzentration: 1.0 mg/ml by UV absorbance at 280 nm
Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Quelle: Chicken
Formulierung: Liquid (sterile filtered)
Application Verdünnung: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Anwendungsbeschreibung: Chicken IgG whole molecule alkaline phosphatase has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
SDS-PAGE of Chicken IgG/IgY Whole Molecule Rhodamine Conjugated (p/n 003-0002). Lane M: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Chicken IgG Whole Molecule Rhodamine Conjugated (p/n 003-0002). Lane 2: Reduced Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Reduced Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Reduced Chicken IgM Whole Molecule (p/n 003-0107). Load: 1 µg per lane. Predicted/Observed size: IgG at 72 and 25 kDa, F(c) at 25 kDa, Fab at 25 kDa, IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.