Human IgG Biotin

Produktübersicht

• Artikelname: Human IgG Biotin
• Artikelnummer: ROC-009-0602
• Hersteller Artikelnummer: 009-0602
• Alternativnummer: ROC-009-0602
• Hersteller: Rockland Immunochemicals
• Kategorie: Antikörper
• Applikation: ELISA
• Konjugation: Biotin
• Alternative Synonym: Human IgG Biotin conjugation, Human immunoglobulin G, Vitamin H, coenzyme R

Produkteigenschaften

• Konzentration: 1.040 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Quelle: Human
• Formulierung: Lyophilized

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:5000 - 1:50,000, IHC: 1:500, WB: 1:1000
• Anwendungsbeschreibung: Human IgG whole molecule Biotin conjugated has been tested in ELISA and can be utilized as a control reagent in both Western Blotting and ELISA format experiments.

Bilder

Binding ability of aptamer PA2/8[S1-58] to living cells ofS. aureus. TwoS. aureusisolates 96-01678 and 05-01042 as well as formaldehyde-fixed cells ofS. aureusCS (Protein A-producing Cowan strain) were used for coating the microtiter plates. Cell suspensions with an OD600=0.7 (1) and further stepwise dilutions (1:5, 1:10, 1:30, 1:100) were applied. 100nM of PA2/8[S1-58] (3'Bio) were added for binding in comparison to 0.13nM IgG (Bio) as positive control for binding to immobilised cells. The blank reaction represents the assay control without any cell coating. Negative controls include reactions with the library (3'Bio) and without biotinylated aptamer or IgG. The results of 2 separate experiments were averaged, whereby each of them was made with 1 (IgG) or 2 (aptamer) replicates of each specific interaction. All experiments contained 1 control reaction per cell type with the library and 3 control reactions per cell type without aptamer or IgG binding reagent. Figure 6. PMID: 27650576.

ELISA Results of Human IgG Whole Molecule Biotin Conjugated. Each well was coated in duplicate with 1.0 µg of Human IgG Whole Molecule Biotin Conjugate. The working dilution is 82,800. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Streptavidin-HRP (p/n S000-03) and TMB substrate (p/n TMBE-1000).

Evaluation of binding ability of aptamer PA2/8 and PA2/8[S1-58] to bacterial cells ofS. aureusby ELONA. Formaldehyde-fixed cells ofS. aureusCS (Protein A-producing Cowan strain) andS. aureusWS (Protein A-deficient Wood46 Strain) as well as living cells ofE. coliK12 as negative control were used for coating the microtiter plates. Cell suspensions with an OD600=0.7 (1) and further stepwise dilutions (1:5, 1:10, 1:30, 1:100, corresponding to OD600=0.14, 0.07, 0.023, 0.007) were applied. 100nM of each biotinylated aptamer variants was added, respectively, for binding. The blank reaction represents the assay control without any cell coating. A positive control for binding to immobilised cells is represented by adding of 0.13nM biotinylated IgG. Negative controls include reactions with the library (3'Bio), non-functional aptamer variant PA2/8[S1-50] (3'Bio), and without biotinylated aptamer or IgG. Averaged results are shown: 2-3 separate experiments per aptamer probe and 11 experiments with IgG, whereby each of them was made with 2-3 replicates of each specific interaction. All experiments contained 1 control reaction per cell type with the library and 3 control reactions per cell type without aptamer probes or IgG. Figure 5. PMID: 27650576.