Human Transferrin Biotin

Produktübersicht

• Artikelname: Human Transferrin Biotin
• Artikelnummer: ROC-009-0634
• Hersteller Artikelnummer: 009-0634
• Alternativnummer: ROC-009-0634
• Hersteller: Rockland Immunochemicals
• Kategorie: Sonstiges
• Applikation: DOT
• Konjugation: Biotin
• Alternative Synonym: Human Transferrin Biotin Conjugation

Produkteigenschaften

• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 7018
• UniProt: Q06AH7
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Quelle: Human
• Formulierung: Lyophilized

Anwendungsbeschreibung

• Anwendungsbeschreibung: Human Transferrin Biotin has been tested in dot blot and is designed for Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.

Bilder

Dot Blot results of Human Transferrin Biotin Conjugated. Antigen: Human Transferrin Biotin Conjugated. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Secondary Antibody: Streptavidin-HRP (p/n S000-03) at 1:40,000 for 30 min at RT. Imaging System ChemiDoc, Filter used: Chemi.

Ex vivo fluorescence imaging of biodistribution of the nanoparticles in an orthotopic mouse model of PBT.Notes:(A) Fluorescent signal observed in the brains of four separate mice with PBT (1-24 hours) injected intravenously (via tail vein) with transferrin-coated Prussian blue nanoparticles (MnPB-ATxRd-Tf). A control mouse with PBT was not injected with nanoparticles. (B) Representative ex vivo fluorescence imaging of organ biodistribution of the nanoparticles at 3 hours post-injection. (C) Histograms quantifying the observed fluorescence biodistribution of the nanoparticles 3 hours post-injection. ROIs for intensity measurements are indicated by white dashed lines in (B).Abbreviations:PBT, pediatric brain tumor, MnPB, manganese-containing Prussian blue, ATxRd, Texas Red-labeled avidin, Tf, transferrin, ROI, region of interest, au, arbitrary units. Human Transferrin Biotin Conjugated (p/n 009-0634). Figure 8. PMID: 24920896.

Dot Blot of Biotin conjugated Human Transferrin. Antigen: Human Transferrin Biotin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 60 min at RT.