Interleukin-4 Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-4 in immunological assays.
Bioactivity of Human Interleukin-4 Recombinant Protein . TF-1 cells were cultured with 0 to 10 ng/mL Human IL-4. Cell proliferation was measured after 72 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Human IL-4 is 0.16-0.24 ng/mL. This value is comparable to expected range of less than 0.2 ng/mL.
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