Platelet Derived Growth Factor-BB Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-BB in immunological assays.
Bioactivity of Mouse Platelet Derived Growth Factor BB Recombinant Protein. Serial dilutions of Mouse PDGF-BB, starting at 100 ng/mL, were added to NIH 3T3 cells. After 40 hours, cell viability was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Mouse PDGF-BB is 0.2-0.3 ng/mL. This value is comparable to the typical expected range of 1-3 ng/mL.
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