Stem Cell Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Stem Cell Factor in immunological assays. Lyophilized with 20 mM sodium phosphate, pH 6.5.
Bioactivity of Mouse Stem Cell Factor Recombinant Protein. Serial dilutions of Mouse SCF, starting at 250 ng/mL, were added to TF-1 cells growing in GM-SCF free media. Cell proliferation was measure after 65 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Mouse SCF is between 5.7- 8.6 ng/mL. The typical range is between 2.5-10 ng/mL.
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