0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Quelle:
Rabbit
Formulierung:
Lyophilized
Application Verdünnung:
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Anwendungsbeschreibung:
Rabbit IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
SDS-PAGE of Rabbit IgG Whole Molecule. Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5µL OPAL Pre-stained Marker (MB-210-0500). Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1µg per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.
Distribution of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) within the pronucleus. Single images were obtained at different positions of the same pronucleus from z-stacks to illustrate the distribution of 5mC and 5hmC. The pattern of distribution of 5mC and 5hmC differs largely, with 5hmC being distributed homogeneously, while 5mC was concentrated in specific DNA regions. 5mC and 5hmC were evaluated in different zygotes. Zygotes were incubated with the non-immune control antibody rabbit IgG (p/n 011-0102). Fig.5. PMID: 28331549.
Lateral flow test results for 58 serum samples, including 51 normal and 7 positive samples. [Symbol legend: (*)P< 0.05, (****)P< 0.0001 (one-way analysis of variance and Fishers least significant difference test).] Mouse anti-human IgG antibody and rabbit IgG [p/n 011-0102] were combined, the mixture was dispensed onto the conjugate pad and dried. Figure 4. PMID: 32323974.
Representative sequences of the 15 identified groups from the NGS pool were screened for their individual binding abilities to Protein A. Comparative SPR-based interaction analyses were performed with the Biacore X100 instrument. Biotinylated aptamers were immobilized via the 5'-end (A) or 3'-end (B) on the streptavidin-modified sensor surface and 1000 nM Protein A was injected for binding. The sensor responses from the end of the binding phases (after 300 s) are shown. In addition, cross-specificities to other proteins were analyzed. Asterisks indicate if certain interactions have not been investigated. Rabbit IgG (p/n 011-0102). Figure 5. PMID: 29495282.
SDS PAGE gel of commercially-purified rabbit Immunoglobulin G (rIgG) and of rIgG papain digestion products Fab and Fc. Lanes A, B and G contain gel markers. Lanes C-F contain products which have been reduced with b-mercaptoethanol. Lanes H-L contain non-reduced products. Each lane contains approximately 35 mg of the following products: Lanes C and H contain papain digested products, Lanes D, I and L contain Fc fragment obtained from protein A purification, Lanes E and J contain whole rIgG, Lanes F and K contain Fab fragment obtained from protein A purification, Whole rIgG (p/n 011-0102), rFc (p/n 011-0103), rFab (p/n 011-0105), rF(ab)2 (p/n 011-0104). Fig. 3. PMID: 12643976.
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