Anti-GST has been tested by western blot and is suitable to be assayed against 1.0 ug of Glutathione-S-Transferase [Schistosoma japonicum] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code 605-4302 and (
Western Blot of Goat anti GST antibody. Lane 1: molecular weight. Lane 2: 1 ug GST reduced. Lane 3: 0.25 ug GST reduced. Lane 4: 1 ug GST non-reduced. Lane 5: 0.25 ug GST non-reduced. Lane 6: molecular weight. Primary antibody: Goat anti GST antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 649 conjugated Donkey anti Goat secondary antibody at 1:10,000 for 1.5 hours at RT. Lane 7: 1 ug GST reduced. Lane 8: 1 ug GST non-reduced. Lane 9: molecular weight. Primary antibody: Goat anti GST antibody at 1:1000 for overnight at 4C. Secondary antibody: Dylight 549 conjugated Donkey anti Goat secondary antibody at 1:10,000 for 1.5 hours at RT. Block: MB-070 overnight at 4C.
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