Bilirubin Oxidase Antibody, Goat, Polyclonal

Produktübersicht

• Artikelname: Bilirubin Oxidase Antibody, Goat, Polyclonal
• Artikelnummer: ROC-100-101-212
• Hersteller Artikelnummer: 100-101-212
• Alternativnummer: ROC-100-101-212
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Fungi
• Immunogen: Bilirubin Oxidase (Myrothecium verrucaria)
• Konjugation: Unconjugated
• Alternative Synonym: goat anti-Bilirubin Oxidase Antibody, Bilirubin Oxidase

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 95 mg/mL by Refractometry
• NCBI: 12737
• UniProt: Q12737
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Myrothecium verrucaria
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:25,000, IHC: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Bilirubin Oxidase has been tested by western blot and is suitable for use in ELISA, western blot, immunohistochemistry, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.

Bilder

Rockland Goat anti Bilirubin Oxidase antibody (200-101-212 lot 6533) was used to detect Bilirubin Oxidase under reducing (R) conditions. A samples of ~1ug of purified protein contained 4% BME and was boiled for 5 minutes and run by SDS-PAGE on a 4-20% acrylamide gel. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT) and Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System