Glutamine Synthetase Antibody, Goat, Polyclonal

Produktübersicht

• Artikelname: Glutamine Synthetase Antibody, Goat, Polyclonal
• Artikelnummer: ROC-100-101-238
• Hersteller Artikelnummer: 100-101-238
• Alternativnummer: ROC-100-101-238
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Bacteria
• Immunogen: Glutamine Synthetase [native from brevibacterium species]
• Konjugation: Unconjugated
• Alternative Synonym: Glutamine synthetase, goat anti-Glutamine Synthetase Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 90 mg/mL by Refractometry
• NCBI: 3345165
• UniProt: Q79VE3
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Microbial
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Anwendungsbeschreibung: Anti-Glutamine Synthetase has been tested by western blot and is suitable to be assayed against 1.0 ug of Glutamine Synthetase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code 605-4302 and (

Bilder

Rockland Goat anti Glutamine Synthetase antibody (200-101-238 lot 8182) was used to detect Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.