Luciferase Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-100-401-137-0100
Artikelname: Luciferase Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-100-401-137-0100
Hersteller Artikelnummer: 100-401-137-0100
Alternativnummer: ROC-100-401-137-0100
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: DOT
Spezies Reaktivität: Bacteria
Immunogen: Luciferase [Photobacterium fischerii]
Konjugation: Unconjugated
Alternative Synonym: rabbit anti-Luciferase Antibody, Alkanal monooxygenase alpha chain, Bacterial luciferase alpha chain, luxA, Alkanal monooxygenase beta chain, Bacterial luciferase beta chain, luxB
Klonalität: Polyclonal
Konzentration: 75 mg/ml by Refractometry
NCBI: 3280269
UniProt: P19907
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Photobacterium fischerii
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:5,000 - 1:25,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
Anwendungsbeschreibung: Luciferase Antibody has been tested by dot blot and is suitable for western blotting, immunohistochemistry, immunoprecipitation, and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Dot Blot results of Rabbit anti-Luciferase Antibody. Dots are Luciferase (Photobacterium fischerii) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: Rabbit anti-Luciferase at 1ug/mL 1hr RT. Secondary Antibody: Goat Anti-Rabbit IgG HRP 611-103-122 at 1:40,000 for 30min RT. Imaged with BioRad ChemiDoc, Chemi filter 5sec exposure.