ALKALINE PHOSPHATASE Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ALKALINE PHOSPHATASE Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-100-401-142
• Hersteller Artikelnummer: 100-401-142
• Alternativnummer: ROC-100-401-142
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Human
• Immunogen: Alkaline Phosphatase [Human Intestine]
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-Alkaline phosphatase antibody, Alkaline phosphatase intestinal antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 85 mg/mL by Refractometry
• NCBI: 248
• UniProt: P09923
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000
• Anwendungsbeschreibung: Anti-Alkaline Phosphatase has been tested by western blot and is suitable to be assayed against 1.0 µg of Alkaline Phosphatase [Human Intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code 611-1302

Bilder

Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (611-1302) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.